Journal: PLoS ONE

Article Title: Iron-Ascorbate-Mediated Lipid Peroxidation Causes Epigenetic Changes in the Antioxidant Defense in Intestinal Epithelial Cells: Impact on Inflammation

doi: 10.1371/journal.pone.0063456

Figure Lengend Snippet: Cells were incubated with Fe/Asc (200 µM/2 mM) and Trolox (0.25 mM) for 6 h at 37°C and/or with 5-AZA (10 µM). The protein expression of Interleukin-6 (IL-6) (A, C) and cyclooxygenase 2 (COX2) (B, D) was determined by western blot as described in Materials and Methods. Results represent the means ± SEM of n = 3 independent experiments. *P<0.05, **P<0.01 vs. controls; # P<0.05, ## P<0.01 vs. Fe/Asc.

Article Snippet: The different primary antibodies were added as follow: 1∶1000 rabbit anti-COX-2 (74 kDa; Novus, Oakville, ON), 1∶5000 goat anti-NF-κB (65 kDa; Santa Cruz Biotechnology, Santa Cruz, CA), 1∶5000 rabbit anti-IκBα (39 kDa; Cell signaling, Beverly, MA) and 1∶500 mouse anti-IL-6 (25 kDa; R&D Systems, Minneapolis, MN).

Techniques: Incubation, Expressing, Western Blot

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression of IL-6 in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, interleukin; MMP, metalloproteinase.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: Expressing, Staining

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) Kaplan-Meier analysis of overall survival for the combination of CD68, CSF-1 and IL-6. (B) Kaplan-Meier analysis of disease-free survival for the combination of CD68, CSF-1 and IL-6. CSF-1, colony-stimulating factor-1; CD, cluster of differentiation; IL, interleukin,

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques:

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) OS in patients with AIS/MIA in the CD68+CSF-1+IL-6+ group compared with other groups, including the CD68-, CD68+CSF-1+IL-6-, CD68+CSF-1-IL-6+ and CD68+CSF-1-IL-6- groups. (B) DFS in patients with AIS/MIA in the CD68+ CSF-1+ IL-6+ group compared with other groups (C) Comparison of OS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. (D) Comparison of DFS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. *Others include the CD68+CSF-1+IL-6- group, the CD68+CSF-1-IL-6+ group, the CD68+CSF-1-IL-6- group and the CD68- group. AIS, adenocarcinoma in situ ; MIA, minimally invasive adenocarcinoma; CD, cluster of differentiation; CSF, colony-stimulating factor; IL, interleukin; OS, overall survival; DFS, disease-free survival.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: In Situ